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Electrical resistance and ion diffusion through mesothelium.

F Bodega¹, L Zocchi, D Cremaschi

¹Istituto di Fisiologia Umana I, Università di Milano, via Mangiagalli 32, 20133 Milan, Italy.

Respiration Physiology

|February 15, 2001

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Phospholipids (PHL) restrict the diffusion of larger solutes across rabbit pericardium mesothelium by reducing intercellular pore size. This phospholipid effect on permeability and electrical resistance was investigated.

Area of Science:

Biophysics
Cell Biology
Physiology

Background:

The mesothelium forms a selectively permeable barrier.
Phospholipids (PHL) are known to influence membrane permeability.

Purpose of the Study:

To investigate the effect of phospholipids (PHL) on the diffusional permeability and electrical resistance of rabbit parietal pericardium mesothelium.
To determine the size selectivity of the mesothelial barrier and the role of PHL in modulating it.

Main Methods:

Measurement of diffusional permeability (P) to Na+, Cl-, and Rb+ in rabbit pericardium specimens with and without PHL.
Electrical resistance (Re) measurements of pericardium specimens, connective tissue, and mesothelium.
Comparison of mesothelium properties with cultured mesothelial cell monolayers.

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Main Results:

PHL decreased diffusional permeability to Na+ but not Cl- or Rb+ (radius > 0.2 nm).
PHL increased the electrical resistance of the mesothelium.
Cultured mesothelial cells showed lower electrical resistance and higher Na+ permeability than in situ mesothelium.

Conclusions:

Phospholipids (PHL) reduce the effective pore size of mesothelium, restricting the diffusion of solutes larger than 0.2 nm.
The mesothelium acts as a significant barrier to ion diffusion, with PHL enhancing this barrier function.
In vitro cultured mesothelial cells may not fully replicate the barrier properties of in situ mesothelium.