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Small-scale bioreactor system for process development and optimization.

P Girard1, M Jordan, M Tsao

  • 1Laboratory of Cellular Biotechnology, Swiss Federal Institute of Technology, Lausanne, Switzerland

Biochemical Engineering Journal
|February 15, 2001
PubMed
Summary
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This study presents an agitated microtiter plate system for efficient cell culture development. The system enhances mass transfer and cell growth, offering a simple, inexpensive, and automatable high-throughput screening method.

Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Cell Culture Technology

Background:

  • Optimizing cell culture conditions is crucial for biopharmaceutical development.
  • Traditional methods often lack high-throughput capabilities for process optimization.
  • Agitation in microtiter plates can improve mass transfer and homogeneity.

Purpose of the Study:

  • To evaluate an agitated 12-well microtiter plate system for cell culture process development.
  • To assess the system's capability for enhancing cell density and pH stability.
  • To establish a simple, inexpensive, and automatable platform for high-throughput cell culture screening.

Main Methods:

  • Utilized a 12-well microtiter plate system with a 2ml working volume and agitation.
  • Controlled pH by adjusting carbon dioxide levels in the gas phase, using phenol red indicator.

Related Experiment Videos

  • Monitored cell growth non-invasively using green fluorescent protein (GFP) expressing cells and a fluorescence plate reader.

    • Main Results:

    • Agitation improved homogeneity and mass transfer, leading to enhanced cell density and pH stability.
    • Spectrophotometric and fluorescence plate readers enabled rapid, precise, and non-invasive measurements.
    • The system demonstrated simplicity, low cost, and potential for automation.

    Conclusions:

    • The agitated microtiter plate system is a viable and efficient tool for cell culture process development.
    • It facilitates high-throughput screening of culture conditions, improving cell yields and stability.
    • This scalable system offers a cost-effective alternative for small-scale bioreactor experiments.