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Related Experiment Videos

Parallel substrate feeding and pH-control in shaking-flasks.

D Weuster-Botz1, J Altenbach-Rehm, M Arnold

  • 1Institute of Biochemical Engineering, Munich University of Technology, Boltzmannstr. 15, 85748, Garching, Germany

Biochemical Engineering Journal
|February 15, 2001
PubMed
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A new intermittent feeding system for shaking flasks enables precise, automated substrate delivery and pH control. This method enhances aerobic cell concentrations and cellular activity compared to traditional batch cultures.

Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Microbial Cultivation

Background:

  • Traditional batch shaking flask cultures have limitations in achieving high cell densities and activities.
  • Bridging the gap between simple batch flasks and complex fed-batch bioreactors is crucial for bioprocess optimization.

Purpose of the Study:

  • To develop and evaluate an automated intermittent feeding system for shaking flasks.
  • To enable precise substrate feeding and pH control in individual shaking flasks.
  • To compare the performance of this system against conventional batch operations.

Main Methods:

  • Development of a system integrating a precise syringe pump, substrate distribution, and miniature solenoid valves for up to 16 shaking flasks.
  • Equipping flasks with pH probes for real-time monitoring and control.

Related Experiment Videos

  • Utilizing a process computer to manage individual feeding profiles and pH-based base/acid addition.
  • Studying physiological effects in a stirred tank reactor using a recombinant E. coli strain.

    • Main Results:

    • Achieved higher concentrations of aerobic cells with enhanced cellular activities in fed-batch, pH-controlled shaking flasks.
    • Demonstrated successful automated and precise control over substrate feeding and pH.
    • The system effectively mimics fed-batch and pH-controlled bioreactor conditions in a shaking flask format.

    Conclusions:

    • The developed intermittent feeding system significantly improves cell cultivation in shaking flasks.
    • This technology offers a scalable and controllable alternative for microbial cultivations, enhancing productivity.
    • Further studies on physiological effects can be conducted using this advanced shaking flask system.