Related Experiment Videos
Crystallization of full-length CysB of Klebsiella aerogenes, a LysR-type transcriptional regulator
K H Verschueren1, C Addy, E J Dodson
1York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO1 5DD, England. koen@ysbl.york.ac.uk
Acta Crystallographica. Section D, Biological Crystallography
|February 15, 2001
Abstract:
CysB is a positive regulator of transcription of genes involved in cysteine biosynthesis in Gram-negative bacteria and belongs to the large family of LysR-type transcriptional regulators. The full-length protein from Klebsiella aerogenes has been crystallized from solutions containing PEG 8000 in the presence and in the absence of the inducer N-acetylserine by the method of vapour diffusion in hanging drops. For the complexed protein different crystal forms appear in the same drops.