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Published on: March 13, 2013
Cell lines from the same cervical carcinoma but with different radiosensitivities exhibit different cDNA microarray
M P Achary1, W Jaggernauth, E Gross
1Department of Radiation Oncology, Albert Einstein College of Medicine of Yeshiva University, and Montefiore Medical Center, Bronx, NY, USA. achary@aecom.yu.edu
Cytogenetics and Cell Genetics
|February 15, 2001
Summary
Identifying radiosensitive cervical cancers could spare patients chemotherapy. This study analyzed gene expression in radioresistant versus radiosensitive cervical cancer cells to find molecular markers for radiation sensitivity.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Chemotherapy combined with radiotherapy improves cervical cancer cure rates.
- Identifying radiosensitive tumors could spare patients from chemotherapy's side effects.
- Current methods cannot distinguish radiosensitive cervical cancers.
Purpose of the Study:
- To identify molecular events and gene expression patterns related to cervical cancer radiosensitivity.
- To hypothesize that specific gene expression levels determine tumor cell sensitivity to ionizing radiation (IR).
- To find potential biomarkers for predicting radiosensitivity in cervical cancer.
Main Methods:
- Compared gene expression profiles of radioresistant and radiosensitive cervical cancer cell lines derived from the same patient tumor.
- Utilized cDNA microarrays to analyze the expression of 5,776 known genes and expressed sequence tags (ESTs).
Main Results:
- Found 52 genes with elevated expression in radioresistant cells (max 4.1-fold increase) and 18 genes overexpressed in radiosensitive cells.
- Identified known genes and novel ESTs associated with differential radiosensitivity.
- Notable overexpressed genes in radioresistant cells included metal-regulatory transcription factor-1 and cytochrome P450 CYP1B1.
- Key overexpressed genes in sensitive cells included transcription factor NF-kappa-B and superoxide dismutase-2.
Conclusions:
- Gene expression profiling can identify molecular differences between radiosensitive and radioresistant cervical cancer cells.
- Specific genes and ESTs show differential expression correlating with response to ionizing radiation.
- These findings provide a basis for developing biomarkers to predict radiosensitivity in cervical cancer patients.
