Three-Dimensional Echocardiography: Precision and Accuracy of Left Ventricular Volume Measurement Using Rotational...

We developed a new, rapid (6 seconds) acquisition technique allowing collection of approximately six through nine apical rotational tomograms for three-dimensional (3-D) echocardiography. To justify an appropriate sampling density for precise and accurate measurement of chamber volumes in left ventricles with complicated shape, we designed a validation study in vitro using six canine heart specimens with irregular, asymmetric left ventricles with known volumes (28.5 to 104.3 ml; mean, 71.2 ml). The number of equally spaced slices were incrementally deleted from the original high resolution scans (48 slices) to 2 slices in 3-D reconstruction. We created subgroups of 48- and 36-, 24- and 16-, 12- and 8-, 6- and 4-, and 3- and 2-component slices to compare left ventricular (LV) volumes measured in 3-D images with different slice resolution with the reference standard measured in the specimen. The accuracy and precision of LV volume were relatively constant in the subgroup of 4- and 6- through 36- and 48-component slices. When the subgroup with 6- and 4-component slices was used, the correlation was r = 0.991, P < 0.0001, root-mean-square percent error of 5.0%, bias of 0.5 +/- 3.7 ml, and interobserver variability of 5.0%. With the reduction in component slices equal or less than three, the accuracy decreased significantly (root-mean-square percent error = 8.1% and bias = -2.0 +/- 5.7 ml) compared with higher slice resolutions. This study demonstrated that 3-D echocardiography using apical rotational techniques can accurately quantify LV volume in the canine heart specimens with irregular shapes with as few as 4-6 axial slices. The rapid 3-D acquisition technique is therefore anticipated to yield precise and accurate LV volumetry.