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Related Experiment Videos

Multiple redundant sequence elements within the fission yeast ura4 replication origin enhancer.

S M Kim1, D Y Zhang, J A Huberman

  • 1Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY, USA. soo-mi.kim@roswellpark.org

BMC Molecular Biology
|February 15, 2001
PubMed
Summary

Fission yeast replication enhancers contain multiple, partially redundant DNA sequence motifs that regulate origin firing. Some motifs overlap with core origin sequences and may bind Origin Recognition Complex (ORC).

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Area of Science:

  • Molecular Biology
  • Genetics
  • Chromosomal Replication

Background:

  • Eukaryotic chromosome origins exhibit variable firing frequencies.
  • A replication enhancer near the fission yeast ura4 gene controls origin firing frequency.
  • Key sequence motifs within this enhancer were previously unmapped.

Purpose of the Study:

  • To localize important sequence motifs within the fission yeast ura4 replication enhancer.
  • To understand the contribution of different sequence segments to enhancer function.
  • To investigate the relationship between enhancer motifs and core origin sequences.

Main Methods:

  • Systematic deletion analysis of the enhancer and adjacent core origin (ars3002).
  • Assaying the functional impact of deletions on origin firing.

Related Experiment Videos

  • Sequence analysis to identify conserved motifs and potential binding sites.

    • Main Results:

    • Multiple sequence motifs within the enhancer contribute to its function in a partially redundant manner.
    • Some enhancer sequences are inhibitory, while others overlap with core origin sequences.
    • Important sequences in both the enhancer and core origin co-localize with AT-rich regions and consensus replication origin motifs, potentially binding Origin Recognition Complex (ORC).

    Conclusions:

    • The ura4 replication enhancer contains multiple sequence motifs, many redundant, that modulate origin function.
    • Some enhancer motifs are similar to those found in core origins.
    • Future research will focus on identifying proteins that bind to these stimulatory sequences.