An ELISA for the quantification of chicken immunoglobulin (IgY) in various liquid media

The development of an assay for the quantification of chicken immunoglobulin (IgY) and data evaluation is being demonstrated. An enzyme immunoassay was established to perform the quality control of IgY purifications from the egg yolk and to control IgY producing cells in vitro. Each step of the test procedure and possible alternatives were described. The assay was characterized by special test parameters (sensitivity, detectability, accuracy, precision, specificity). Moreover, this simple and fast enzyme immunoassay is a special supplement to the alternative antibody production from egg yolk.