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Related Experiment Videos

[Monoclonal antibodies: Comparative methods for in vitro production]

René W. Fischer1, Philippe C. Ferber

  • 1Laboratorium für Biochemie 1, ETH Zürich, CH-Zürich.

ALTEX
|January 1, 1992
PubMed
Summary
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The ascites method for monoclonal antibody production is being replaced by alternative techniques like static cultures and bioreactors. These methods offer viable options for researchers, eliminating the need for the ethically problematic "ascites-mouse".

Area of Science:

  • Biotechnology
  • Immunology
  • Cell Biology

Background:

  • The ascites method, while effective for monoclonal antibody production, causes significant animal distress and is increasingly restricted.
  • Ethical concerns and regulatory changes necessitate the exploration of alternative antibody production techniques.

Purpose of the Study:

  • To investigate and compare alternative methods for monoclonal antibody production.
  • To assess the applicability of various cell culture techniques in a research laboratory setting.
  • To provide guidance on selecting appropriate methods to replace the ascites-mouse model.

Main Methods:

  • Comparison of static tissue culture, spin cultures, roller cultures, and bioreactor systems.
  • Evaluation of antibody yield and feasibility for different production scales.

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  • Assessment of practical implementation in a standard research laboratory.

    • Main Results:

    • All investigated alternative methods demonstrated feasibility for monoclonal antibody production.
    • Static cultures, spin cultures, roller cultures, and bioreactors provide scalable options for antibody generation.
    • A suitable alternative exists for any required antibody quantity, negating the need for the ascites method.

    Conclusions:

    • Alternative methods effectively replace the ascites-mouse for monoclonal antibody production.
    • Researchers can select from various techniques based on desired antibody yield and laboratory capabilities.
    • The transition away from the ascites method is achievable and ethically sound.