[A new method for the study of flow of material into surviving blood vessel segments]

One of the most important questions arising in experiment on surviving segments of vessels is the question of flow of material into the vessel wall through the intima or adventitia. To make a distinction is important for the interpretation of results of in vitro experiments. By using a "tissuephil" tested already in practical surgery wound-adhesive free of any noxious effect, authors could form segments of vessels, having an only intimal or adventitial surface. Using this technique the flow of blood into the wall through intima or adventitia could be investigated separtely. At in vitro experiments most part of the labelled material flows into the vessel wall through the adventitia. This fact can be explained by the presence of microinjuries of the adventitia caused by the removing of the surrounding fat tissue. It is believed that only the flow through the intima gives an information about the condition of the vessels. Flow through the adventitia is mostly artificial.