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Updated: Aug 14, 2026

Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
Expression of the major heat shock proteins DnaK and GroEL in Streptococcus pyogenes: a comparison to Enterococcus
M S Laport1, A C de Castro, A Villardo
1Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Departamento de Microbiologia Médica, CCS, Bloco I, Cidade Universitária, 21491-590, Rio de Janeiro, RJ, Brazil.
Abstract:
One of the outstanding problems in the field of heat shock response has been to elucidate the mechanism underlying the induction of heat shock proteins (HSPs). In this work, we initiate an analysis of the expression of heat shock groEL and dnaK genes and their promoters in S. pyogenes. The synthesis of total cellular proteins was studied upon transfer of a log-phase culture from 37 degrees C to 42 degrees C by performing 5-min pulse-labeling experiments with (35)S-Met. The heat shock responses in the pathogenic Gram-positive cocci, Enterococcus faecalis and Staphylococcus aureus, were also analyzed.
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