Transcription factors: global and detailed views

During the past year, much progress has been made in understanding the structural basis of transcriptional regulation. Low-resolution electron microscopy structures of general transcription factor complexes have shed light on their global organization. These results are complemented by the structural and biochemical analysis of individual general transcription factors. High-resolution crystal structures of sequence-specific transcription factors still yield unexpected results. Detailed analysis of DNA recognition by different family members of the same class of sequence-specific transcription factors shows considerable variations in the way they interact with DNA.