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Simultaneous catalysis and product separation by cross-linked enzyme crystals
M Leisola1, J Jokela, J Finell
1Laboratory of Bioprocess Engineering, Helsinki University of Technology, P.O. Box 6100, FIN- 02015 HUT, Helsinki, Finland.
Biotechnology and Bioengineering
|February 17, 2001
Summary
Crystalline cross-linked enzymes, xylose isomerase and xylanase, were used in packed-bed reactors for simultaneous catalysis and separation. This approach efficiently separated L-arabinose, L-ribulose, and L-ribose, and processed xylotetraose.
Area of Science:
- Biocatalysis
- Enzyme Engineering
- Separation Technology
Background:
- Enzymes like xylose isomerase and xylanase are crucial for carbohydrate processing.
- Simultaneous reaction and separation can enhance process efficiency.
Purpose of the Study:
- To investigate the use of crystalline cross-linked xylose isomerase (CLXI) and xylanase (CLX) in packed-bed reactors.
- To achieve simultaneous catalytic reaction and separation of substrates and products.
Main Methods:
- Packed-bed reactor studies using crystalline cross-linked xylose isomerase (CLXI) and xylanase (CLX).
- Analysis of reaction mixtures for substrate and product concentrations.
Main Results:
- CLXI facilitated simultaneous isomerization of L-arabinose to L-ribulose and epimerization to L-ribose, achieving variable product fractions.
- CLX efficiently hydrolyzed xylotetraose to xylotriose and xylobiose, and further to xylobiose and xylose.
Conclusions:
- Packed-bed reactors with CLXI and CLX enable efficient, simultaneous biocatalytic conversion and separation.
- This integrated approach shows promise for industrial applications in carbohydrate processing.