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Updated: Aug 1, 2026

Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
Published on: January 29, 2020
Axolotl MHC class II beta chain: predominance of one allele and alternative splicing of the beta1 domain
V Laurens1, C Chapusot, M del Rosario Ordonez
1CNRS-UMR 5548, Développement et Communication Chimique, Groupe Immunologie Comparée, Université de Bourgogne, Dijon, France. vlaurens@u-bourgogne.fr
Abstract:
The axolotl MHC is composed of multiple polymorphic class I loci linked to class II B loci. In this report, evidence of the existence of one class II B locus (Amme-DAB) that codes for two different transcripts is given. A 2.1-kb transcript is translated to a complete beta chain and a shorter transcript of 1.8 kb encodes a molecule lacking the beta1 domain. For two complete class II B mRNA synthesized, up to one mRNA devoid of the beta1 domain is synthesized. Alternative splicing involving a peptide binding domain at a class II B locus evidenced in axolotl (Ambystoma mexicanum) is also observed for A. tigrinum, the tiger salamander. Very little variability is found among various axolotl MHC class II B cDNA sequences, and the same allele is obtained from inbred and wild axolotls. The transcription of one MHC class B locus in two class II B isoforms in thymic cells and in splenic lymphocytes may shed new light on the well-known deficient immune responder state of the axolotl.
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