Separation of chiral 4-substituted imidazole derivatives by cyclodextrin-modified capillary electrophoresis

Enantiomeric separations by cyclodextrin-modified capillary electrophoresis of chiral carbamates containing a 4-substituted imidazole heterocycle, pharmacologically acting as histamine H3-receptor antagonists, were carried out. Various cyclodextrins (alpha-, beta- and substituted beta-cyclodextrins) were investigated. Baseline resolution was achieved with six out of 11 compounds. Heptakis-2,3,6-trimethyl-beta-cyclodextrin modified buffers seemed to be most efficient in separating most of the investigated compounds. The optical purity of one pair of enantiomers was determined.