Related Experiment Video
Updated: Aug 7, 2026

On-Chip Endothelial Inflammatory Phenotyping
Published on: July 21, 2012
Localized activation of m-calpain in migrating human umbilical vein endothelial cells stimulated by shear stress
H Ariyoshi1, N Yoshikawa, Y Aono
1Department of Surgery II, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565, Japan. ariyoshi@surg2.med.osaka-u.ac.jp
Abstract:
Using a parallel-plate flow-chamber and confocal laser scanning microscopy (CLSM), we studied the mode of cytoskeletal reorganization in migrating HUVECs stimulated by shear stress. Activation of m-calpain associated with a change in the spatial distribution of cytoplasmic ionized Ca2+ concentration ([Ca2+](i)) was studied. Shear stress (10 dyne/cm(2)) caused migration and decrease in the F-actin content of HUVECs. Migrating individual HUVECs showed the lamellipodium formed in the direction of cell migration, in which [Ca2+](i) elevated to 148 +/- 12 nM in a localized fashion. We found the appearance of activated m-calpain in the local area of the migrating HUVECs, which was associated with a decrease in the amounts of pp125FAK and ezrin. The localized rise in [Ca2+](i) might be closely related to morphological change to regulate the direction of cell migration induced by shear stress through localized activation of m-calpain.
More Related Videos
09:20The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
Published on: October 31, 2016
09:10Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch
Published on: April 21, 2023
Related Concept Videos
Overview of Cell-Matrix Interactions
Cell-matrix's Response to Mechanical Forces
Anchoring junctions mechanically attach a cell to the...