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Related Experiment Videos

Pacing redistributes glycogen within the developing myocardium.

X Lyon1, L Kappenberger, D Sedmera

  • 1Division of Cardiology, University Hospital, BH 16.309, 1011 Lausanne-CHUV, Switzerland. xlyon@hola.hospvd.ch

Journal of Molecular and Cellular Cardiology
|February 22, 2001
PubMed
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Electrical pacing in embryonic chick hearts rapidly depleted glycogen stores in the atrium and conotruncus, not the ventricle. This suggests pacing-induced myocardial remodeling is linked to altered energy metabolism.

Area of Science:

  • Cardiovascular Physiology
  • Metabolic Regulation
  • Developmental Biology

Background:

  • Electrical pacing at physiological rates can induce myocardial remodeling, affecting workload, blood flow, and oxygen consumption.
  • The impact of pacing on energy-producing pathways, particularly glycogen metabolism, in the developing heart remains under-investigated.
  • Hypertrophy and increased workload are known to influence glycolysis and substrate utilization (glucose vs. fat oxidation).

Purpose of the Study:

  • To investigate the modifications in glycogen metabolism within the paced embryonic chick heart.
  • To determine if electrical pacing affects glycogen content in different heart regions (atrium, ventricle, conotruncus).
  • To correlate changes in glycogen metabolism with observed hemodynamic and remodeling alterations.

Main Methods:

Related Experiment Videos

  • Utilized an in ovo embryonic chick heart model (stage 22HH).
  • Applied asynchronous and intermittent ventricular pacing at a physiological rate for 24 hours.
  • Assessed glycogen content in atrium, ventricle, and conotruncus of paced and sham-operated hearts.
  • Derived hemodynamic parameters (end-diastolic volume, stroke volume) via computerized image analysis.

Main Results:

  • Paced hearts exhibited significant decreases in glycogen content in the atrium (1.48±0.40 vs. 0.84±0.34) and conotruncus (0.75±0.28 vs. 0.42±0.23) compared to sham controls.
  • Ventricular glycogen content did not show a significant decrease.
  • Pacing led to a 34% decrease in end-diastolic volume and a 44% decrease in stroke volume.

Conclusions:

  • Rapid glycogen depletion occurs in heart regions remote from the pacing site (atrium, conotruncus).
  • These metabolic changes are associated with regional workload alterations and myocardial remodeling induced by pacing.
  • Highlights the critical coupling between metabolic pathways and myocardial remodeling in ectopically paced hearts.