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Related Experiment Videos

Penicillin-binding proteins in Leptospira interrogans.

A Brenot1, D Trott, I Saint Girons

  • 1Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France.

Antimicrobial Agents and Chemotherapy
|February 22, 2001
PubMed
Summary

The Leptospira interrogans ponA and pbpB genes, encoding penicillin-binding proteins (PBPs) 1 and 3, were characterized. Both genes produce functional PBPs, showing high similarity to E. coli homologs.

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Area of Science:

  • Microbiology
  • Molecular Biology

Background:

  • Penicillin-binding proteins (PBPs) are essential targets for beta-lactam antibiotics.
  • Leptospira interrogans is a bacterial pathogen responsible for leptospirosis.

Purpose of the Study:

  • To isolate and characterize the ponA and pbpB genes in Leptospira interrogans.
  • To determine the functional activity of the encoded penicillin-binding proteins.

Main Methods:

  • Gene isolation and sequencing of ponA and pbpB from two L. interrogans strains.
  • Bioinformatic analysis comparing L. interrogans PBP sequences to E. coli homologs.
  • In vitro synthesis and ampicillin modification of L. interrogans PBP 1 and PBP 3 in E. coli.

Main Results:

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  • Low sequence variation was observed between PBP genes from different L. interrogans serovars.
  • Deduced PBP 1 and PBP 3 protein sequences shared over 50% similarity with E. coli homologs.
  • Both ponA and pbpB genes encode functional penicillin-binding proteins.

    • Conclusions:

    • The ponA and pbpB genes in L. interrogans are conserved and encode functional PBPs.
    • These findings contribute to understanding antibiotic resistance mechanisms in Leptospira.