[Electrophoretic profiles of proteins and glycoproteins of chick embryo fibroblasts during development]
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Chick embryo fibroblast proteins and glycoproteins change during development. High molecular weight glycoproteins increase from day 8 to 12, suggesting cell surface alterations and potential links to tumor cells.
Area of Science:
- Developmental Biology
- Cell Biology
- Biochemistry
Background:
- Chick embryo fibroblasts (CEFs) are a model system for studying cellular development.
- Understanding protein and glycoprotein dynamics is crucial for comprehending developmental processes.
Purpose of the Study:
- To investigate quantitative modifications in proteins and glycoproteins of CEFs during embryonic development.
- To characterize cell surface components and their changes over time.
Main Methods:
- Polyacrylamide disc gel electrophoresis in sodium dodecyl sulfate (SDS-PAGE) was employed.
- Fluorescamine labeling and trypsin sensitivity assays were used to identify cell surface components.
Main Results:
- Two high molecular weight glycoproteins (250,000 and 200,000 Da) showed quantitative changes.
- These glycoproteins increased significantly between the 8th and 12th days of development.
- Their levels remained stable from the 12th to the 16th day of development.
Conclusions:
- The identified glycoproteins are likely cell surface components.
- Developmental changes in these glycoproteins may offer insights into cell surface regulation.
- The findings provide a basis for comparing CEF development with tumor cell characteristics.
Abstract:
The variations of proteins and glycoproteins of Chick embryo fibroblasts are studied during development. This investigation is carried out using polyacrylamide disc gel electrophoresis in SDS. Two glycoproteins of high apparent molecular weight (250,000 and 200,000) undergo quantitative modification: they increase from the 8th to 12th day of development and then remain unchanged to the 16th day. They are cell surface components as suggested by fluorescamine labelling and trypsin sensitivity. The results are discussed in terms of relationship between tumor- and embryo cells.