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Changes in detrusor and urinary growth factors according to detrusor function after partial bladder outlet
J Chul Kim1, S Il Seo, Y Hyun Park
1Department of Urology, Catholic University of Korea College of Medicine, Seoul, South Korea.
Objectives:
To investigate the changes in detrusor and urinary growth factors according to detrusor function after partial bladder outlet obstruction in the rat. Growth factors have been reported to play a role in the remodeling of the bladder after obstruction.
Methods:
Female rats (n = 40) were used and divided into three groups: sham-operated control, 2-week mild obstruction, and 2-week severe obstruction. The voided urine was collected and the detrusor contractility to electrical stimulation recorded. mRNA expression and the urinary concentration of transforming growth factor beta-1 (TGF-beta1) and basic fibroblast growth factor (bFGF) were analyzed.
Results:
The contractile response to electrical stimulation was increased in the mild obstruction group (P <0.05) but decreased in the severe obstruction group (P <0.05). The detrusor mRNA expression of TGF-beta1 in the mild obstruction group was not different from that in the control group. However, in the severe obstruction group, the mRNA expression of TGF-beta1 increased. The detrusor mRNA expression of bFGF was gradually increased in the mild and severe obstruction groups. The urinary concentration of TGF-beta1 in the mild obstruction group was not significantly different from that in the control group. However, in the severe obstruction group, the concentration of TGF-beta1 was significantly increased (P <0.05). The concentration of urinary bFGF was too small to compare the results in each group.
Conclusions:
Growth factors such as TGF-beta1 and bFGF change in mRNA expression according to bladder function, and urinary TGF-beta1 reflects the change of detrusor mRNA. Thus, changes in TGF-beta1 may be a marker to evaluate detrusor function.