Related Experiment Videos
[76Br]Bromodeoxyuridine PET in tumor-bearing animals
O Gardelle1, U Roelcke, P Vontobel
1Section of Diagnostic Imaging, Veterinary School, University of Zurich, 8057, Zürich, Switzerland.
Nuclear Medicine and Biology
|February 22, 2001
Summary
Positron emission tomography with [76Br]BUdR is not suitable for imaging tumor cell proliferation. Mathematical corrections for bromide washout did not yield reliable tumor tracer retention data.
Area of Science:
- Oncology
- Radiochemistry
- Nuclear Medicine
Background:
- 5-bromodeoxyuridine (BUdR) is an in vitro method for assessing tumor cell proliferation.
- Positron emission tomography (PET) offers in vivo imaging capabilities.
Purpose of the Study:
- To evaluate the utility of [76Br]BUdR with PET for in vivo measurement of tumor cell proliferation.
- To assess the tissue and plasma kinetics of [76Br]BUdR in tumor-bearing animal models.
Main Methods:
- Tumor-bearing animals were administered [76Br]BUdR.
- Positron emission tomography was used to monitor tracer kinetics.
- Mathematical corrections were applied to account for the slow washout of the [76Br]bromide metabolite.
Main Results:
- Specific tumor tracer retention was low or negligible after correction for bromide washout.
- The corrected tracer retention did not correlate with independent measures of tumor proliferation.
- [76Br]BUdR exhibited unfavorable kinetic characteristics for proliferation imaging.
Conclusions:
- The kinetic properties of [76Br]BUdR limit its application in PET imaging of tumor proliferation.
- This tracer is currently unsuitable for quantitative in vivo proliferation assessment.