[Development of ELISAs for endocrine disrupting chemicals]

Six kinds of ELISA systems were developed for the quantitative analysis for endocrine disrupting chemicals, such as bisphenol A(BPA), diethylhexylphthalate (DEHP), dibutylphthalate(DBP), alkylphenol(AP), alkylphenol ethoxylate(APE) and chlorophenols(CP). The detection limit of ELISAs were 5, 150, 200, 10, 20 and 2 ng/ml, respectively. The specificities of monoclonal antibodies used for ELISA systems were confirmed with the cross-reaction test using several compounds which have structural resemblance.