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Embryonic stem cells and gene targeting.

B Ledermann1

  • 1Institute of Laboratory Animal Science, University of Zurich-Irchel, Switzerland. diamant@ltk.unizh.ch

Experimental Physiology
|February 24, 2001
PubMed
Summary
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Gene targeting allows precise modification of mouse genomes using homologous recombination in embryonic stem cells. This review details essential tools and methods for analyzing gene function in vivo.

Area of Science:

  • * Molecular Biology
  • * Genetics
  • * Developmental Biology

Background:

  • * Gene targeting enables precise genome alteration via homologous recombination.
  • * Application in murine embryonic stem cells allows predetermined germ-line modification in mice.
  • * This technology is crucial for analyzing gene function in vivo.

Purpose of the Study:

  • * To review essential tools and methodologies for gene targeting developed over the past decade.
  • * To highlight the importance of embryonic stem cell lines and genetic background.
  • * To discuss the current state of gene targeting in non-murine species.

Main Methods:

  • * Homologous recombination for allele exchange.
  • * Utilization of murine embryonic stem cells.

Related Experiment Videos

  • * Review of established and emerging gene targeting techniques.

    • Main Results:

    • * Comprehensive overview of gene targeting tools and methodologies.
    • * Emphasis on critical factors like stem cell lines and genetic background.
    • * Discussion of gene targeting advancements in various species.

    Conclusions:

    • * Gene targeting is a powerful technique for in vivo gene function analysis.
    • * The review provides a valuable resource for researchers in the field.
    • * Future directions and applications of gene targeting are highlighted.