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Transgenic technology in farm animals--progress and perspectives.

E Wolf1, W Schernthaner, V Zakhartchenko

  • 1Institute of Molecular Animal Breeding, Gene Centre, Ludwig-Maximilian University, Munich, Germany. ewolf@lmb.uni-muenchen.de

Experimental Physiology
|February 24, 2001
PubMed
Summary
This summary is machine-generated.

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Gene transfer in farm animals enhances product traits and disease resistance. Cloning offers a promising alternative for efficient, targeted genetic modification in livestock.

Area of Science:

  • Agricultural Science
  • Biotechnology
  • Genetics

Background:

  • Gene transfer applications in farm animals aim to improve product quality, disease resistance, and create models for research.
  • Traditional methods like DNA microinjection have low efficiency and random integration issues.

Purpose of the Study:

  • To review current gene transfer techniques in farm animals.
  • To highlight challenges and advancements in creating genetically modified livestock.

Main Methods:

  • Review of existing literature on gene transfer methods including DNA microinjection, sperm-mediated gene transfer, retroviral vectors, and cloning.
  • Analysis of efficiency, integration, and potential applications of each method.

Main Results:

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  • DNA microinjection is inefficient with random integration.
  • Sperm-mediated gene transfer is under development.
  • Retroviral vectors show efficiency in transgenic cattle production.
  • Cloning offers a viable route for targeted genetic modification.

Conclusions:

  • While gene transfer offers benefits, challenges in efficiency and integration persist.
  • Cloning from cultured cells presents a significant advancement for genetic modification in farm animals.