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Updated: Aug 3, 2026
10:06
An Efficient Method for Adenovirus Production
Published on: June 10, 2021
[Simplified method for gene transfer and expression by recombinant adenoviruses]
Y Maruyama1, T Nagao, H Kurose
1Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
|February 24, 2001
Summary
Generating recombinant adenoviruses for gene delivery is now simpler and more efficient. New methods overcome traditional challenges, making adenovirus technology more accessible for research and therapeutic applications.
Area of Science:
- Molecular Biology
- Virology
- Biotechnology
Context:
- Recombinant adenoviruses are key tools for gene delivery in scientific research.
- Traditional methods for generating these viruses are inefficient and complex, hindering their widespread application.
- Existing techniques often involve difficult homologous recombination in mammalian cells, requiring extensive time and expertise.
Purpose:
- To present simplified and more efficient systems for producing recombinant adenoviruses.
- To provide a practical guide for generating recombinant adenoviruses using improved methodologies.
- To address the limitations of traditional adenovirus vector generation.
Summary:
- This work introduces advanced adenoviral technologies, including novel vectors and improved production systems utilizing homologous recombination in E. coli.
- The described methods streamline the generation of recombinant adenoviruses, overcoming the inefficiencies associated with older techniques.
- A practical manual is provided to facilitate the easier and more efficient production of these viral vectors.
Impact:
- Accelerates the use of adenovirus technology for gene transfer in scientific and potentially clinical settings.
- Enhances the accessibility of recombinant adenovirus generation for researchers globally.
- Facilitates advancements in gene therapy and molecular biology research through improved viral vector production.
