Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Automated crystal mounting and data collection for protein crystallography.

S W Muchmore1, J Olson, R Jones

  • 1Department of Structural Biology, Abbott Park, Illinois 60064, USA.

Structure (London, England : 1993)
|February 24, 2001
PubMed
Summary

An automated system for protein crystallography automates crystal handling and X-ray data collection. This innovation enhances efficiency for structural genomics and drug design research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Method for Electrical Stimulus Artifact Removal Exploiting Neural Refractoriness: Validation by Contrasting Cathodic and Anodic Stimulation.

bioRxiv : the preprint server for biology·2024
Same author

Implementation of a drive cylinder for low collisional experiments on magnetic reconnection.

The Review of scientific instruments·2023
Same author

Effect of RMGI Roughness and Dentin Bonding Primer on Shear Bond Strength of Sandwich-type Restorations.

Operative dentistry·2023
Same author

Scintillation light detection in the 6-m drift-length ProtoDUNE Dual Phase liquid argon TPC.

The European physical journal. C, Particles and fields·2022
Same author

Prospects for beyond the Standard Model physics searches at the Deep Underground Neutrino Experiment: DUNE Collaboration.

The European physical journal. C, Particles and fields·2021
Same author

Ion Heating and Flow Driven by an Instability Found in Plasma Couette Flow.

Physical review letters·2021

Area of Science:

  • Structural Biology
  • Biophysics
  • Crystallography

Background:

  • Advances in X-ray sources, optics, and detectors have reduced data acquisition times.
  • Increased availability of synchrotron beam lines allows for faster data collection per crystal.
  • Higher throughput necessitates automated solutions for unattended data collection.

Purpose of the Study:

  • To develop an automated system for efficient diffraction data collection in protein crystallography.
  • To enable unattended mounting, alignment, data collection, and storage of protein crystals.

Main Methods:

  • Development of an automated system for storing frozen protein crystals.
  • Sequential mounting and alignment of crystals to an X-ray beam.
  • Automated collection of complete diffraction data sets.

Related Experiment Videos

Main Results:

  • The developed system automates the entire process of diffraction data collection.
  • This automation significantly increases the efficiency of protein crystallography experiments.
  • The system facilitates unattended data acquisition, crucial for high-throughput studies.

Conclusions:

  • Automated data collection systems are essential for modern protein crystallography.
  • This technology enhances efficiency in structural genomics and structure-directed drug design.
  • The system supports advanced applications like screening by X-ray crystallography.