[Interactions among stimulators of bone resorption in organ culture]

The labeled 45Ca long bone shafts of 19 day fetal rats in organ culture was used in the study. Actions and interactions between various concentrations of LPS, TNF, LTA and PGE were quantitatively analysed by sample channels ratio method. The results indicated that they could all induce bone resorption in organ culture and show significance of differences assessed using student's test (P < 0.05). Interacts between LPS and TNF, LPS and PGE, TNF and PGE were greater than the sum of the individual responses when added together (the expection value), LPS and LTA, TNF and LTA were equal to the expection value, but LTA and PGE were less than the expection value. The result supported that the interactions among these stimulators of bone resorption were important in the pathogenesis of bone loss in periodontal disease.