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Lack of corneal toxicity of interferon Alpha-2b administered subconjunctivally after sclerectomy
1Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Purpose:
To evaluate the corneal toxicity of subconjunctival injection interferon alpha-2b at filtering bleb after sclerectomy in white rabbits.
Methods:
Eight rabbits which had been performed sclerectomy were randomly divided into two groups. Each group consisted of four rabbits. Eight eyes in group 1 were subconjunctivally received interferon alpha-2b 5 x 10(5) IU/0.2 ml into filtering bleb from the edge of the filtering site immediately after operation and every postoperative day. The other eight eyes in group 2 were injected with 0.2 ml normal saline. All of the eyes underwent daily examination by slip-lamp microscopy and direct ophthalmoloscopy. Sodium fluorescein was used to assess corneal epithelial integrity. On day 3, 4, 7 and 14, every two rabbits (group 1 and 2 each, respectively) were killed and removed cornea immediately to take examination of the viability of corneal endothelium by dual staining with typan blue and alizanin red S.
Results:
No sign of toxicity in corneal epithelium and endothelium were found following continuously injection interferon alpha-2b at filtering bleb 3-14 days totally for selected doses.
Conclusion:
Subconjunctivally administered interferon alpha-2b at filtering bleb after glaucoma filtering surgery may be a safe drug-applied method.