[Cloning and endonuclease digestion of lon gene from Streptomyces ansochromogenes]

Streptomyces ansochromogenes is a nikkomycin producer which has typical life cycle of Streptomyces differentiation on glucose asparagine medium. The lon gene product is an ATP-dependent serine proteinase. The lon gene might have some connection with physiological differentiation and also with the morphological differentiation in S. coelicolor. The lon-like gene of S. ansochromogenes was identified by hybridization and the lon-like gene in 4.6 kb PstI fragment was cloned. The physical map of this fragment and the localization of the target gene were preliminarily studied.