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[The modulation effect of granulocyte-colony stimulating factor on the mdr1/P-gp expression in acute myeloid leukemic
Objective:
To investigate the correlation between cell differentiation and mdr1 gene expression in acute myeloid leukemic(AML) cells.
Methods:
The modulation effect of granulocyte-colony stimulating factor(G-CSF) on the mdr1 gene expression in AML cells was studied in vitro by using polymerase chain reaction(PCR), in vitro cell culture, immunocytochemical and MTT assays.
Results:
AML cells retained the ability to respond to the differentiation stimulus. Expressions of P-glycoprotein(P-gp) and mdr1 mRNA were downregulated in differentiated cells, meanwhile, a significant decrease of DNR IC50 in the AML cells with down-expression mdr1 gene was revealed and their sensitivity to anti-cancer drugs increased.
Conclusion:
Leukemic cell differentiation was closely correlated with mdr1 expression. It might be an effective method to overcome drug resistance of leukemic patient by using differentiation inducing agents.
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