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[Application of comparative genomic hybridization to hyperdiploid acute lymphoblastic leukemia]
1Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
Objective:
To evaluate the implication of comparative genomic hybridization (CGH) in leukemia study.
Methods:
Genomic abnormalities in 14 ALL patients were assayed by CGH, and the results were compared with those of conventional karyotype analysis.
Results And Conclusion:
Regional and/or whole chromosome over-representation was found to be more frequent than under-representation (43 gains versus 6 losses), the most common gains involved being chromosomes 21 and X. Comparison between the results of CGH and conventional R-banding analysis showed that: 1. In 2 cases with trisomy, both the methods gave identical results. 2. In 8 cases, both the results were consistent excepting for minor discrepancies. 3. In 3 cases, including 2 each with triploidy and tetraploidy respectively, and one with chimeric karyotype of normal/+22, the results from the two methods were discrepant.