[Isolation and characterization of promoters from Phanerochaete chrysosporium]

Promoter-probe vector pSUPV8 was used to clone promoters from Phanerochaete chrysosporium directly in Escherichia coli. Six hygromycin B-resistant recombinants were obtained and two inserted fragments of them were sequenced. The results indicated that they contain several sequences similar to eukaryotic cis regulatory elements. Only pCH6 could transform P. chrysosporium into hygromecin B resistance. PCR and dot hybridization analysis indicated that it was introduced into P. chrysosporium successfully and leaded to the HmB-resistance. The results demonstrated that HmB-resistant gene (hph) could be used as an ideal reporter gene for P. chrysosporium transformation.