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A random amplified polymorphic DNA polymerase chain reaction technique that differentiates between Neospora species
J A Spencer1, A K Witherow, B L Blagburn
1Department of Pathobiology, College of Veterinary Medicine, Auburn University, Alabama 36849, USA.
The Journal of Parasitology
|February 24, 2001
Summary
Neospora caninum and the newly identified Neospora hughesi are distinct coccidial parasites. The random amplified polymorphic DNA polymerase chain reaction technique effectively differentiates these two Neospora species.
Area of Science:
- Veterinary Parasitology
- Molecular Diagnostics
- Protozoology
Background:
- Neospora caninum, a coccidial parasite, infects various mammals.
- Neospora hughesi, a new species, is linked to equine protozoal myeloencephalitis in horses.
Purpose of the Study:
- To differentiate between Neospora caninum and the newly identified Neospora hughesi.
- To establish a molecular method for distinguishing these closely related parasites.
Main Methods:
- Utilized random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR).
- Compared genetic profiles of N. caninum and N. hughesi isolates.
Main Results:
- The RAPD-PCR technique successfully differentiated N. caninum from N. hughesi.
- Distinct DNA profiles were observed between the two species.
Conclusions:
- Neospora hughesi is a distinct species from Neospora caninum.
- RAPD-PCR is a reliable method for distinguishing between these two important veterinary parasites.