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Updated: Jul 16, 2026
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Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Capillary electrochromatography with physically and dynamically absorbed stationary phases
1National Chromatographic R & A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences. zouhfa@mail.dlptt.ln.cn
Electrophoresis
|February 24, 2001
Summary
Adsorption in capillary electrochromatography (CEC) can be leveraged to create stationary phases, offering a simpler alternative to chemical synthesis. This review explores dynamically adsorbed stationary phases for separating various solutes and enantiomers.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Adsorption is typically viewed as a detrimental effect in capillary electrophoresis (CE) and capillary electrochromatography (CEC).
- However, adsorption phenomena can be strategically utilized for stationary phase preparation in CEC.
Purpose of the Study:
- To review the application of physically and dynamically adsorbed stationary phases in CEC.
- To highlight the advantages of adsorption-based stationary phase preparation over chemical synthesis.
Main Methods:
- Review of literature on CEC with dynamically adsorbed stationary phases.
- Discussion of separation mechanisms for charged solutes and stationary phases in CEC.
Main Results:
- Demonstration of the successful separation of acidic, basic, and neutral solutes using dynamically adsorbed stationary phases.
- Presentation of enantiomeric separations achieved with these CEC methods.
Conclusions:
- Dynamically adsorbed stationary phases offer a convenient and effective approach for CEC.
- Exploiting adsorption provides a simpler alternative for optimizing CEC stationary phases.
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