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Characterisation of Jack fruit lectin
1Biochemistry Department, SSMC, Dhaka.
Bangladesh Medical Research Council Bulletin
|February 24, 2001
Summary
Jack fruit lectin (JFL) from Artocarpus Heterophyllus seeds comprises two active subunits, 12.5 and 15.0 KDa. Both subunits retain lectin activity, with the smaller subunit exhibiting greater agglutinating potential.
Area of Science:
- Biochemistry
- Molecular Biology
- Phytochemistry
Background:
- Jack fruit seed extract contains Jack fruit lectin (JFL), a protein with diverse biological properties.
- A comprehensive analysis of JFL's specific characteristics has been previously lacking.
Purpose of the Study:
- To conduct a detailed investigation into the properties of Jack fruit lectin (JFL).
- To characterize the subunits of JFL and assess their individual biological activities.
Main Methods:
- Extraction and purification of JFL using affigel galactosamine-agarose chromatography.
- Analysis of JFL's subunit composition using Native PAGE (ND-PAGE) followed by SDS-PAGE.
- Isolation and separate biological activity assessment of eluted subunit bands.
Main Results:
- JFL resolved into two distinct trimeric subunits of 12.5 KDa and 15.0 KDa.
- Both isolated subunits retained significant lectin activity.
- The smaller subunit (12.5 KDa) displayed higher agglutinating activity, potentially due to its relative abundance.
Conclusions:
- JFL is composed of at least two biologically active subunits.
- Each subunit contributes to the overall lectin activity of JFL.
- The differential agglutinating activity suggests functional variations between the subunits.