Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Creatinephosphokinase in human diploid cell lines.

M Macek, H Tomásová, J Hurych

    Advances in Experimental Medicine and Biology
    |January 1, 1975
    PubMed
    Summary

    Creatine phosphokinase (CPK) activity was detected in various human cultivated cells, showing consistent levels across developmental stages and tissues. This finding supports prenatal diagnosis for CPK-related metabolic disorders.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The sensitivity of SARS-CoV-2 antigen tests in the view of large-scale testing.

    Epidemiologie, mikrobiologie, imunologie : casopis Spolecnosti pro epidemiologii a mikrobiologii Ceske lekarske spolecnosti J.E. Purkyne·2021
    Same author

    Production of proteolytic enzymes in mast cells, fibroblasts, vascular smooth muscle and endothelial cells cultivated under normoxic or hypoxic conditions.

    Physiological research·2010
    Same author

    In vitro hypoxia increases production of matrix metalloproteinases and tryptase in isolated rat lung mast cells.

    Physiological research·2007
    Same author

    Association of tumour necrosis factor alpha variants with the CF pulmonary phenotype.

    Thorax·2005
    Same author

    Inhalation trauma in burns, cytology and biochemical findings.

    Acta chirurgiae plasticae·2004
    Same author

    Polymorphisms in the mannose binding lectin gene affect the cystic fibrosis pulmonary phenotype.

    Journal of medical genetics·2004

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Developmental Biology

    Background:

    • Creatine phosphokinase (CPK) is an enzyme crucial for cellular energy metabolism.
    • Understanding CPK activity in different cell types is important for diagnosing metabolic disorders.

    Purpose of the Study:

    • To investigate the presence and levels of CPK activity in various human cultivated cells.
    • To explore the potential for prenatal diagnosis of CPK-related conditions.

    Main Methods:

    • Cultivation of cells from amniotic fluid, thymus, spleen, pericardium, umbilical cord, skin, and muscle.
    • Assay of CPK activity in these cell cultures.
    • Comparison of CPK activity across different developmental stages (embryonal, fetal, children) and post-mortem samples.

    Main Results:

    • CPK activity was consistently detected in all investigated cultivated cell strains.
    • No significant differences in CPK activity were observed between different tissues or developmental stages.
    • Average CPK activity was measured at 224 ± 68 IU/mg N.
    • CPK activity remained stable in post-mortem cultures.
    • A potential relationship between collagen synthesis and CPK activity is suggested.

    Conclusions:

    • CPK activity is present and consistent across a wide range of human fetal and postnatal cells.
    • The comparable CPK activity in amniotic fluid cells to other fetal cultures indicates potential for prenatal diagnostic applications.
    • This research paves the way for prenatal investigation of CPK pathophysiology and inborn errors of metabolism.

    Related Experiment Videos