Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Apoptosis and intestinal barrier function.

C Bojarski1, K Bendfeldt, A H Gitter

  • 1Department of Gastroenterology, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200 Berlin, Germany.

Annals of the New York Academy of Sciences
|February 24, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High prevalence and functional effects of serum antineuronal antibodies in patients with gastrointestinal disorders.

Neurogastroenterology and motility·2018
Same author

Campylobacter jejuni impairs sodium transport and epithelial barrier function via cytokine release in human colon.

Mucosal immunology·2017
Same author

Campylobacter jejuni impairs sodium transport and epithelial barrier function via cytokine release in human colon.

Mucosal immunology·2017
Same author

Tricellulin is regulated via interleukin-13-receptor α2, affects macromolecule uptake, and is decreased in ulcerative colitis.

Mucosal immunology·2017
Same author

Investigations of the excitation energy transport mechanism in donor-acceptor systems.

Journal of fluorescence·2013
Same author

Direct and reverse energy transport in systems of monomers and imperfect traps: Monte Carlo simulations.

Journal of fluorescence·2013
Same journal

Multiomics Profiling During Autoimmune Demyelination Highlights a Complex Regulatory Role for Ataxin-1 in B Cells.

Annals of the New York Academy of Sciences·2026
Same journal

Global Trends in Light Pollution and Their Relationship With Socioeconomic Factors.

Annals of the New York Academy of Sciences·2026
Same journal

Wired for Corruption: Inter-Brain Synchrony Encodes Bribery-Related Value Information and Predicts Bribery Agreement.

Annals of the New York Academy of Sciences·2026
Same journal

LM-YOLO: A Lightweight Multi-Scale Enhanced Model for Forest Smoke Detection Using Unmanned Aerial Vehicles.

Annals of the New York Academy of Sciences·2026
Same journal

Polyrhythm Perception and Production: A Scoping Review.

Annals of the New York Academy of Sciences·2026
Same journal

DARTS-CNN-BiLSTM: Intelligent Fault Diagnosis for Computer Numerical Control Machine Tool Feed System.

Annals of the New York Academy of Sciences·2026
See all related articles

Camptothecin induces apoptosis in intestinal cells, significantly weakening the epithelial barrier. This study reveals how apoptosis impacts intestinal barrier function, paving the way for further research.

Area of Science:

  • Gastrointestinal physiology
  • Cell biology
  • Molecular signaling

Background:

  • Apoptosis induction pathways in the gastrointestinal tract are partially understood.
  • The impact of apoptotic signals on intestinal barrier function remains largely unknown.

Purpose of the Study:

  • To investigate the effect of camptothecin-induced apoptosis on HT-29/B6 intestinal cell monolayers.
  • To characterize the influence of apoptosis on epithelial barrier function.

Main Methods:

  • Utilized camptothecin to induce apoptosis in HT-29/B6 cell monolayers.
  • Measured transepithelial electrical resistance (TER) and paracellular permeability using [3H]mannitol flux.
  • Assessed single-cell apoptosis conductance via conductance scanning technique.
  • Employed Ussing chamber technique for barrier function analysis.

Related Experiment Videos

Main Results:

  • Camptothecin significantly decreased transepithelial resistance, indicating barrier disruption.
  • Increased fluxes of the paracellular marker [3H]mannitol were observed.
  • Camptothecin elevated the apoptotic rate and single-cell apoptosis conductance.
  • Demonstrated significant epithelial barrier defects in response to camptothecin-induced apoptosis.

Conclusions:

  • Camptothecin is a potent inducer of apoptosis in HT-29/B6 cells, causing notable intestinal barrier defects.
  • The study provides a foundation for investigating other cytokines, such as TGF-beta, in apoptotic conditions affecting the intestinal barrier.