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Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
Published on: October 15, 2014
Rapid recovery and identification of anthrax bacteria from the environment
J L Kiel1, J E Parker, J L Alls
1Directed Energy Bioeffects Division, Air Force Research Laboratory, Brooks Air Force Base, Texas 78235, USA. johnathan.kiel@brooks.af.mil
Abstract:
Bacillus anthracis has been recognized as a highly likely biological warfare or terrorist agent. We have designed culture techniques to rapidly isolate and identify "live" anthrax from suspected environmental release. A special medium (3AT medium) allows for discrimination between closely related bacilli and non-pathogenic strains. Nitrate was found to be a primary factor influencing spore formation in Bacillus anthracis. Nitrate reduction in anthrax is not an adaptation to saprophytic environmental existence, but it is a signal to enhance environmental survival upon the death of the anthrax host, which can be mimicked in culture.
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