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Organization of the rat PACAP gene
1Department of Anatomy and Neurobiology, University of Vermont College of Medicine, Burlington, Vermont 05405, USA. slw@salus.uvm.edu
Annals of the New York Academy of Sciences
|February 24, 2001
Abstract:
The rat PACAP gene has been isolated, cloned, and sequenced. The gene organization is very similar to that of the mouse and human PACAP genes. An analysis of the 5'-UTR in proPACAP mRNAs indicates that multiple transcripts are generated by using different splice sites within exons 1A, 1B, and 2 to form the 5'-UTR. Analysis of PACAP promoter activity in PC12 cells suggests that forskolin responsiveness of the gene is dependent on elements located between -77 and -413 bp from the transcription start site.