Improvement of hatchability of chicken eggs injected by blastoderm cells

In our first experiment, we studied the effect of injection method of blastoderm cells (BC) into the subgerminal cavity of White Leghorn embryos on hatchability of chicken chimeras. Freshly laid eggs were injected through a hole made in the equatorial plane of the eggshell (Method A). In Method B, eggs were stored pointed end down for 5 to 7 d prior to injection, and a hole was cut in the blunt end of the eggshell. An advantage of Method B was that the early embryonic mortality was reduced (P < or = 0.01) and resulted in higher hatchability (41.0%; 43/105) than Method A (9.8%; 14/143). In the second experiment, we studied chicken hatchabililty as influenced by windowing (no hole, Group 1; hole in the equatorial plane, Group 2; hole in the blunt end of egg, Groups 3 and 4) and egg turning (Groups 1 and 4) or not (Groups 2 and 3) during incubation. The hatchability percentages were as follows: 67.9 (Group 1) 0.0, (Group 2) 23.3, (Group 3), and 56.8 (Group 4). A statistically significant difference (P < or = 0.05) was noted between Group 1 or 4 and the other groups. We found no statistically significant differences in the weight changes (g) but did note certain differences in the egg weight loss (%) among different egg treatments. In the third experiment, we investigated the influence of origins of BC donors: Rhode Island Red (RIR), Barred Plymouth Rock (BPR), and Green-legged Partridgelike (GP) on hatchability of putative and somatic chimera chickens. The hatchability of chimeras was dependent on the adequate assortment of BC of the donor and ranged from 7.4% (RIR) to 56.1% (GP). In the case of BC injection of the GP breed, good hatchability was accompanied by very high percentage (86.9; 20/23) of somatic chimeras.