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Considerations for the evaluation of renal function in genetically engineered mice.
1Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Ohio 45267-0576, USA. lorenzjn@uc.edu
Current Opinion in Nephrology and Hypertension
|February 24, 2001
Summary
Functional genomics in mice requires adapted techniques for studying cardiorenal physiology. Recent advances enable precise measurements of blood pressure, renal function, and single-nephron processes in mice.
Area of Science:
- Physiology
- Genomics
- Cardiorenal Function
Background:
- Functional genomics utilizes transgenic and gene-targeting techniques.
- Mice are the primary model organism, necessitating specialized physiological evaluation methods.
- Adapting techniques from rats to mice for cardiorenal studies presents unique challenges.
Purpose of the Study:
- To review recent advances in measuring murine cardiorenal physiology.
- To highlight techniques applicable to cellular, tissue, organ, and whole-animal levels in mice.
- To discuss limitations and considerations for applying established methods in mouse models.
Main Methods:
- Adaptation of established physiological measurement techniques for mice.
- Measurement of blood pressure and renal blood flow.
- Assessment of whole kidney and single-nephron function, including GFR and transport.
Main Results:
- Successful adaptation of techniques for measuring diverse cardiorenal variables in mice.
- Advancements in whole kidney and single-nephron functional assessments.
- Detailed review of methods for blood pressure, renal blood flow, electrolyte excretion, GFR, and tubuloglomerular feedback.
Conclusions:
- Sophisticated techniques are available for evaluating mouse cardiorenal physiology.
- Understanding species-specific adaptations is crucial for accurate murine physiological studies.
- Recent advances facilitate comprehensive functional genomics research in mice.