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Updated: Sep 11, 2026

Surface Spreading and Immunostaining of Yeast Chromosomes
Published on: August 9, 2015
Organization of highly repeated sequences in surface-spread pachytene chromosomes of rye
N Cuñado1, J Barrios, J L Santos
1Departamento de Genética, Facultad de Biología, Universidad Complutense, Madrid, Spain.
Abstract:
A method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) associated with fluorescence in situ hybridization (FISH) has been applied to analyze the location and organization of five different highly repeated DNA sequences in rye. Our observations indicate that, depending on the type of sequence, the chromatin displays different types of organization. Telomeric sequences were seen tightly associated with the SC while other repetitive DNA sequences were found to form loops that are associated with SCs only at their bases. On the contrary, the FISH signal of a centromeric satellite had a granular appearance, reflecting that the hybridization occurs only with parts of the chromatin loops.
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