Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Experimental Escherichia coli respiratory infection in broilers

S M Peighambari1, R J Julian, C L Gyles

  • 1Department of Pathobiology, Ontario Veterinary College, University of Guelph, Canada.

Avian Diseases
|February 24, 2001
PubMed
Summary

Researchers established an optimal model for avian colibacillosis reproduction in broiler chickens. A 4-day interval between infectious bronchitis virus (IBV) and avian pathogenic Escherichia coli challenges effectively mimics natural disease characteristics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trend of Changes in the Titer of Antibody against Avian Influenza Virus H9n2 during Raising Period in Vaccinated and Unvaccinated Broiler Farms in Qazvin Province, Iran: A Cohort Study.

Archives of Razi Institute·2020
Same author

Molecular characterization of haemagglutinin-neuraminidase gene among virulent Newcastle disease viruses isolated in Iran.

Iranian journal of veterinary research·2019
Same author

Co-circulation of genetically distinct groups of avian paramyxovirus type 1 in pigeon Newcastle disease in Iran.

Avian pathology : journal of the W.V.P.A·2016
Same author

PCR-based diagnosis, molecular characterization and detection of atypical strains of avian Chlamydia psittaci in companion and wild birds.

Avian pathology : journal of the W.V.P.A·2013
Same author

Isolation and characterization of a very virulent Infectious bursal disease virus from turkey.

Acta virologica·2009
Same author

Methods for genotyping verotoxin-producing Escherichia coli.

Zoonoses and public health·2009

Area of Science:

  • Veterinary Medicine
  • Avian Pathology
  • Microbiology

Background:

  • Avian colibacillosis, caused by pathogenic Escherichia coli, is a significant disease in broiler chickens.
  • Experimental reproduction is crucial for understanding pathogenesis and developing control strategies.

Purpose of the Study:

  • To determine optimal conditions for experimentally reproducing avian colibacillosis in broiler chickens using aerosolized pathogenic Escherichia coli.
  • To establish a reliable model that mimics the clinical and pathological features of naturally occurring disease.

Main Methods:

  • Broiler chickens (2-4 weeks old) were challenged intranasally with infectious bronchitis virus (IBV).
  • Following a 4-day interval, chickens were exposed to aerosolized avian pathogenic Escherichia coli (O2 or O78 strains) in a Horsfall unit.

Related Experiment Videos

  • Disease severity was assessed based on mortality, airsacculitis, pericarditis, perihepatitis, and E. coli recovery.

    • Main Results:

    • An interval of 4 days between IBV and E. coli challenges was optimal, regardless of chicken age (8 or 20 days).
    • The model typically produced 50%-80% airsacculitis and low mortality, with minimal pericarditis or perihepatitis.
    • E. coli alone caused minimal disease, while IBV alone did not induce lesions. Aerosol challenge via a cone-shaped chamber increased airsacculitis rates.

    Conclusions:

    • The established model (IBV followed 4 days later by aerosolized pathogenic E. coli) effectively reproduces avian colibacillosis with characteristic low mortality and high airsacculitis.
    • This model provides a valuable tool for studying avian colibacillosis pathogenesis and evaluating interventions in broiler chickens.