Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein nitration.

W N Kuo1, J M Kreahling, V P Shanbhag

  • 1Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, FL 32114, USA.

Molecular and Cellular Biochemistry
|February 24, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Denitration of peroxynitrite-treated proteins by 'protein nitratases' from rat brain and heart.

Molecular and cellular biochemistry·2000
Same author

Modification of proteins and polynucleotides by peroxynitrite.

Cytobios·1999
Same author

Denitration of peroxynitrite-treated proteins by "protein nitratases" from dog prostate.

Biochemistry and molecular biology international·1999
Same author

Long-term changes in tyrosine phosphorylation of the abundant nuclear proteins during granulocytic differentiation of HL-60 cells.

Cellular and molecular life sciences : CMLS·1999
Same author

Constitutive nitric oxide synthase in Saccharomyces cerevisiae.

Biochemistry and molecular biology international·1998
Same author

Soluble guanylate cyclase in Saccharomyces cerevisiae.

Biochemistry and molecular biology international·1998
Same journal

High glucose-induced mitochondrial fission promotes Müller cell activation via suppression of the Hippo pathway.

Molecular and cellular biochemistry·2026
Same journal

Correction to: Estradiol inhibits vascular endothelial cells pro-inflammatory activation induced by C-reactive protein.

Molecular and cellular biochemistry·2026
Same journal

Galectin-3, transforming growth factor beta 1, and brain natriuretic peptide in cardiac remodeling under hyperlipidemic and hyperglycemic stress.

Molecular and cellular biochemistry·2026
Same journal

Ellagic acid from the traditional Chinese medicinal herb Scutellaria barbata may accelerates apoptosis in hepatic stellate cells during liver fibrosis via inhibiting miR-182-5p.

Molecular and cellular biochemistry·2026
Same journal

GeneQuantify: a web-based tool for qPCR gene expression and copy number variation analysis.

Molecular and cellular biochemistry·2026
Same journal

NCAPG reprograms glycolytic and lipid metabolism by sustaining glycerophospholipid flux in small-cell lung cancer.

Molecular and cellular biochemistry·2026
See all related articles

This study found varied levels of nitrated tyrosine in proteins, suggesting it

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Post-Translational Modifications

Background:

  • Nitration of tyrosine residues is a key post-translational modification.
  • Nitric oxide (NO) and peroxynitrite (PN) are involved in cellular signaling.
  • Understanding protein nitration is crucial for signal transduction research.

Purpose of the Study:

  • To investigate the presence and extent of endogenous protein nitration in various commercially available proteins and enzymes.
  • To explore variations in protein nitration in different biological contexts, including rat hearts and non-mammalian organisms.
  • To assess the functional impact of nitration on enzyme activity, specifically cAMP-dependent protein kinase (PKA).

Main Methods:

  • Western blot analysis without reducing agents to detect endogenously nitrated tyrosine.

Related Experiment Videos

  • Testing a diverse panel of commercially sourced proteins and enzymes.
  • Analysis of crude extracts from hypertensive and normaltensive rat hearts.
  • Enzymatic activity assays to determine kinetic parameters (Km) of PKA before and after peroxynitrite treatment.

    • Main Results:

    • Strong, moderate, and weak nitrotyrosine immunoreactivity was observed across various proteins, with some lacking it entirely.
    • Differential nitration patterns were detected in histone fractions from hypertensive versus normaltensive rat hearts.
    • Nitrotyrosine immunoreactivity was present in non-mammalian organisms (E. coli, S. cerevisiae, T. vulgaris).
    • Peroxynitrite treatment significantly increased the apparent Km of PKA for cAMP, altering its enzymatic activity.

    Conclusions:

    • Protein tyrosine nitration is a widespread post-translational modification occurring in vivo.
    • The extent of nitration varies significantly among different proteins and biological sources.
    • Discriminative protein nitration plays a role in nitric oxide/peroxynitrite-regulated cellular signaling pathways.
    • Post-translational modification via nitration can impact protein function and enzyme kinetics.