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Updated: Oct 9, 2026

Characterizing Electron Transport through Living Biofilms
Published on: June 1, 2018
Electron-transfer pathways between redox enzymes and electrode surfaces: reagentless biosensors based on
W Schuhmann1, H Zimmermann, K Habermüller
1Analytische Chemie-Elektroanalytik & Sensorik, Ruhr-Universität Bochum, D-44780 Bochum, Germany. woschu@anachem.ruhr-uni-bochum.de
Abstract:
Based on previous results which showed that quinohemo-protein alcohol dehydrogenase (QH-ADH) entrapped within polypyrrole is able to directly transfer electrons via the conducting polymer to the electrode surface, the electron-transfer properties of this multi-cofactor enzyme adsorbed and covalently-bound to self-assembled thiol monolayers and bare electrode surfaces has been investigated more closely. While the dissolved enzyme is able to transfer electrons to the electrode via heme c as well as via the more deeply buried PQQ (fast adsorption-chemical reaction-desorption mechanism), an orientation of adsorbed QH-ADH on hydrophobic electrode surfaces, as well as of adsorbed and covalently bound QH-ADH on negatively-charged thiol monolayers could be observed. In these cases the heme c units are pointing towards the electrode surfaces resulting in an optimised direct ET rate.
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