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Updated: Oct 9, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
Enzyme assisted ensiling of alfalfa with enzymes by solid substrate fermentation
J Schmidt1, G Szakács, E Cenkvári
1Department of Animal Nutrition, Pannon University of Agricultural Sciences, H-9200 Mosonmagyaróvár, Hungary.
Abstract:
A crude enzyme preparation, obtained by solid substrate fermentation (SSF) with a Gliocladium spp. and added at the 5% level to wilted or non-wilted alfalfa, improved the fermentation characteristics and stability of alfalfa silages as effectively as commercial preparations, Novo-Nordisk Celluclast 1.5 L and Viscozyme 120 L, applied at the 0.025% level. The effective dose of the crude enzyme costs about one-fourth of the cost of the commercial enzymes.
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