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Role of macrophages in experimental malaria: VII--studies on adoptive transfer of macrophages
1Malaria Research Centre (ICMR), 52-Nanak Enclave, Radio Colony, Delhi-110 009.
Abstract:
Adoptive transfer of purified macrophages harvested from normal, Plasmodium berghei infected and latent/cured mice and also macrophages exposed to parasites in vitro were carried out to see the role of macrophages in transferring immunity against P. berghei infection. Macrophages obtained from mice having high parasitaemia at a dose of one million cells/animal showed significant increase in survival period (SP) and K values, compared to controls. Macrophages exposed to low parasite density conferred significant K values only. There was a decrease in prepatent period (PP) in the animal which received macrophages from animals cured 7-11 months compared to controls. The adoptive transfer studies with macrophages conditioned in vitro to parasite contributed towards increased protection of host against P. berghei as expressed by K values only. These studies showed that the macrophages harvested from infected mice were capable of acting as immunogen against P. berghei infection.
Insights
Macrophages from Plasmodium berghei-infected mice can transfer immunity. Adoptive transfer of these immune cells significantly improved host survival and protection against malaria parasite infection.
Area of Science:
- Immunology
- Infectious Diseases
- Parasitology
Background:
- Macrophages play a crucial role in the immune response to Plasmodium berghei infection.
- Understanding how macrophages mediate immunity is vital for developing effective malaria control strategies.
Purpose of the Study:
- To investigate the role of macrophages in conferring immunity against Plasmodium berghei infection through adoptive transfer.
- To evaluate the protective effects of macrophages harvested from infected, cured, or in vitro-exposed mice.
Main Methods:
- Adoptive transfer of purified macrophages from normal, infected, and cured mice into recipient mice.
- In vitro exposure of macrophages to Plasmodium berghei parasites.
- Assessment of host protection using survival period (SP) and K values, and prepatent period (PP).
Main Results:
- Macrophages from mice with high parasitemia significantly increased SP and K values in recipients.
- Macrophages exposed to low parasite density conferred significant K values only.
- Adoptive transfer of macrophages from cured mice led to a decreased prepatent period.
- In vitro-conditioned macrophages enhanced host protection, indicated by K values.
Conclusions:
- Macrophages harvested from Plasmodium berghei-infected mice act as immunogens, capable of transferring immunity.
- Macrophage-derived immunity is crucial for host defense against Plasmodium berghei infection.
- The state of macrophage activation influences the degree of protection conferred.
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