An in vitro study of the cytotoxicity of two root canal sealers

The purpose of this in vitro study was to determine the cytotoxicity of two root canal sealing materials (AH26 and AH-Plus). This cytotoxicity test (agar diffusion test) was conducted based on the procedures described in the International Organization for Standardization. The biological reactivity of a mammalian monolayer, L929 mouse fibroblast cells, in response to the tested agents was determined. After the 48-h observation period, the cell cultures exposed to the test articles discs for AH26 and AH-Plus exhibited severe reactivity (grade 4). The positive control article exhibited moderate reactivity (grade 3). No signs of reactivity (grade 0) were noted for the negative control article or the negative control discs. The tested samples of AH26 and AH-Plus are considered cytotoxic and do not meet the requirement of the agar diffusion test. Similar cytotoxicity results have been found in the literature for AH26 and other root canal sealing cements.