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A study on the morphological changes of the rat mandibular bone with TEA CO2 laser
Y Nakamura1, M Hossain, H Watanabe
1Department of Endodontics, School of Dentistry, Showa University, Tokyo, Japan.
Objective:
The purpose of this study was to investigate the morphological changes of the bone structure induced by transversely excited, atmospheric TEA pressure CO2 laser irradiation. Moreover, the healing process at 3 weeks was also observed.
Summary Background Data:
It has been demonstrated that dental hard tissue can be removed by a long pulse of TEA CO2 laser irradiation with minimal thermal damage. However, there are few studies on the morphological changes of the TEA CO2 laser on bone tissue.
Methods:
The TEA CO2 laser was irradiated on the surface of the rat mandibular bone under the following irradiation conditions: wavelength, 10.6 microns; output, 95 mJ/pulse; pulse repetition rate, 1 Hz; irradiation time, 7.5 microseconds/shot; spot size, 0.8 x 1.5 mm; energy density, 7.9 J/cm2. Histological and scanning electron microscopic examinations were performed.
Results:
In the stereoscopic examination, a defect with clean-cut margins was produced. In the light microscopic examination, a basophilic line at the bottom of the defect was noted. Examining the defect with SEM, a smear-like appearance and cone structures were found. In the histological examination, a healing process by formation of new bones at the junction site was recognized.
Conclusions:
These findings suggest that it is possible to remove bone tissues by TEA CO2 laser irradiation. However, a particle-like structure associated with a basophilic line and melted surface indicated that some thermal damage was produced during the laser irradiation.