Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MAP kinase activation in macrophages.

K M Rao1

  • 1Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia, USA. mir8@cdc.gov

Journal of Leukocyte Biology
|February 24, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence of hypertension, its correlates and awareness among adult tribal population of Kerala state, India.

Journal of postgraduate medicine·2013
Same author

Seizure duration over ect sessions: influence of spacing ects.

Indian journal of psychiatry·2011
Same author

Ect induced EEG seizure: validity of duration estimation by last spike.

Indian journal of psychiatry·2011
Same author

EEG delta band spectral power: differneces between unilateral and bilateral ect seizures.

Indian journal of psychiatry·2011
Same author

Effectiveness of macrogol and talcum base formulations of a new insect repellent N, N-diethyl phenyl acetamide.

International journal of cosmetic science·2009
Same author

Laparoscopic ovum pick-up followed by in vitro embryo production for the reproductive rescue of aged goats of high genetic value.

Reproduction, fertility, and development·2007
Same journal

Correction to: IL-27 signaling negatively regulates FcɛRI-mediated mast cell activation and allergic response.

Journal of leukocyte biology·2026
Same journal

Autofluorescence enables detection and quantification of EETosis in human leukocyte cultures.

Journal of leukocyte biology·2026
Same journal

Cold Atmospheric Plasma as an Immunomodulator: Suppression of T-cell Hyperactivation and Graft-versus-Host Disease via Redox Regulation.

Journal of leukocyte biology·2026
Same journal

Distinct lymphocyte immune signatures to nivolumab and recombinant IL-7 ex vivo in patients with sepsis.

Journal of leukocyte biology·2026
Same journal

Eosinophil Essentials: Identification and Assessment of Activity.

Journal of leukocyte biology·2026
Same journal

Immune cell landscape reveals 5 immune-related subtypes and molecular characteristics with prognostic and therapeutic implications in pan-cancer.

Journal of leukocyte biology·2026
See all related articles

Stimulating macrophages activates mitogen-activated protein kinases (MAPKs). Research highlights differences in MAPK activation between cell lines and primary cells, emphasizing the need for primary cell studies.

Area of Science:

  • Immunology
  • Cell Biology
  • Signal Transduction

Background:

  • Macrophages are crucial immune cells activated by various stimuli.
  • Mitogen-activated protein kinases (MAPKs) are key signaling molecules involved in cellular responses.
  • Understanding MAPK activation pathways in macrophages is essential for immunology and disease research.

Purpose of the Study:

  • To explore the mechanisms of MAPK activation in macrophages.
  • To investigate the roles of specific signaling molecules in TNF-alpha-induced MAPK activation.
  • To compare MAPK activation in different macrophage populations and cell types.

Main Methods:

  • Review of existing literature on MAPK signaling pathways.
  • Discussion of molecular players like CD14, Toll receptors, TRADD, FADD, MADD, TRAFs, ASK1, GCK, HPK1, and GCKR.

Related Experiment Videos

  • Consideration of data from knockout mice and mutant cell lines.

    • Main Results:

    • Lipopolysaccharide (LPS)-induced MAPK activation involves CD14 and Toll receptors.
    • Tumor necrosis factor-alpha (TNF-alpha)-induced MAPK activation engages death domain proteins and TRAFs.
    • MAPK activation pathways can differ significantly between cell lines and primary macrophages.
    • Macrophages from different compartments exhibit distinct receptor and signaling molecule expression.

    Conclusions:

    • Primary macrophages are crucial for studying MAPK signal transduction pathways.
    • Extrapolation of MAPK activation data from cell lines to primary cells may be inaccurate.
    • Differences in MAPK activation reflect distinct macrophage phenotypes and functions.